Table 1

Binding of [3H]tiotidine in COS-7 cells

COS-7 transfectionKdBmax
nM fmol/mg
pCEFL19  ± 51.5  ± 0.1 (3)
pCEFL-HA-H2r21  ± 611.2  ± 1.1 (3)*
pCEFL-H2r22  ± 412.4  ± 1.5 (3)*

The K d and B max values were calculated using the equation for one binding site. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The K d and B maxvalues of the pCEFL and the pCEFL-HAH2r and pCEFL-H2r were compared using an unpaired t test and found to be significantly different (* p < 0.01).