COS-7 transfection | EC50 | Rmax |
---|---|---|
nM | % | |
pCEFL-HA-H2r | 48 ± 10 (5) | 102 ± 12 |
+ pCEFL-GRK2 | 137 ± 18 (3)* | 52 ± 11* |
+ pCEFL-GRK3 | 142 ± 23 (3)* | 52 ± 10* |
+ pCEFL-GRK5 | 48 ± 13 (3) | 105 ± 15 |
+ pCEFL-GRK6 | 42 ± 16 (3) | 98 ± 18 |
The EC50 and R max values were calculated from the data in Fig. 4B using the equation for dose response curve. The table shows the mean ± S.E.M.; number of determinations (n) is in parentheses. The EC50 andR max values of the pCEFL-HA-H2r and the pCEFL-GRK2 and GRK3 were compared using an unpaired t test and found to be significantly different (* p < 0.01).