Additive (Test Compound) | Net [3H]Uridine Transport |
---|---|
% control | |
Pyrimidine nucleosides, nucleobases, ribose and dilazep | |
None | 100 |
Uridine | 2 ± 0.8 |
5-Fluorouridine | 1 ± 0.6 |
5-Bromouridine | 7 ± 5 |
5-Iodouridine | 9 ± 7 |
5-Methyluridine | 38 ± 3 |
2′-Deoxyuridine | 3 ± 1.4 |
3′-Deoxyuridine | 99 ± 7 |
2′,3′-Dideoxyuridine | 101 ± 5 |
5-Fluoro-2′-deoxyuridine | 18 ± 2 |
5-Bromo-2′-deoxyuridine | 56 ± 3 |
5-Iodo-2′-deoxyuridine | 69 ± 5 |
5-Fluoro-5′-deoxyuridine | 80 ± 5 |
Thymidine | 74 ± 3 |
Cytidine | 94 ± 7 |
2′-Deoxycytidine | 96 ± 6 |
Uracil | 68 ± 3 |
5-Fluorouracil | 82 ± 3 |
Thymine | 85 ± 2 |
Cytosine | 99 ± 5 |
Ribose | 97 ± 4 |
Dilazep | 88 ± 3 |
Purine nucleosides and nucleobases | |
Inosine | 4 ± 2.8 |
2′-Deoxyinosine | 5 ± 4 |
Guanosine | 1 ± 0.8 |
2′-Deoxyguanosine | 3 ± 2 |
Adenosine | 2 ± 1.7 |
2′-Deoxyadenosine | 2 ± 1.3 |
2′,3′-Dideoxyadenosine | 96 ± 3 |
2-Chloroadenosine | 15 ± 3 |
2-Chloro-2′-deoxyadenosine | 72 ± 4 |
7-Deazaadenosine | 100 ± 2 |
Adenine | 98 ± 3 |
Hypoxanthine | 96 ± 5 |
Guanine | 101 ± 2 |
Arabinonucleosides and arabinose | |
1-β-d-Arabinofuranosyluracil | 70 ± 4 |
1-β-d-Arabinofuranosylcytosine | 102 ± 6 |
9-β-d-Arabinofuranosyladenine | 31 ± 2 |
2-Fluoro-9-β-d-arabinofuranosyladenine | 84 ± 3 |
9-β-d-Arabinofuranosylhypoxanthine | 20 ± 2 |
Arabinose | 99 ± 3 |
Rates of transport of 10 μM [3H]uridine obtained from 3 to 4-min time courses similar to those shown in Fig. 3 were conducted in Na+-containing and Na+-free transport buffer in the absence and presence of 1 mM test compounds listed. Uridine transport values in the absence of additives (controls) ranged from 0.1 ± 0.005 pmol/μl cell water/s to 0.12 ± 0.013 pmol/μl cell water/s. Net transport rates are the Na+-dependent component expressed as percentage of control values and are means ± S.D. of triplicate determinations.