Receptor Source | GABA | CGP7930 EC50 | Hill coefficient | n |
---|---|---|---|---|
μM | μM | |||
Native (rat cortex membranes) | 1 | 5.37 ± 1.02 | 1.2 ± 0.15 | 6 |
20 | 3.65 ± 0.70 | 1.5 ± 0.11 | 6 | |
Recombinant (expressed in CHO cells) | 1 | 4.60 ± 0.83 | 1.00 ± 0.08 | 3 |
20 | 1.87 ± 0.10 | 1.29 ± 0.12 | 3 |
Concentration-response curves for CGP7930 were measured with recombinant or native receptor preparations in the presence of a low (1 μM) and a higher (20 μM) concentration of GABA, as shown in Fig. 4and as described under Materials and Methods. The data shown are means ± S.E.M. from n independent experiments.