Microarray analysis
| Identified Gene | Description | Average Fold δ Expression |
|---|---|---|
| Brain-expressed HHCPA78 homolog | A human 1α,25-dihydroxy-vitamin D3 hydroxylase | ↑ 4.3 (± 0.83) |
| Keratin type 1 cytoskeleton 14 | Cytoskeleton protein | ↓ 5.1 (± 0.54) |
| Calmegin | Putative testis-specific chaperon. | ↑ 2.5 (± 0.32) |
| Cyclin D3 | Promotes progression through the G1 phase of the cell cycle. | ↓ 2.5 (± 0.19) |
| H-cadherin | Cell adhesion molecule | ↓ 2.5 (± 0.40) |
| Gravin | A kinase scaffold protein | ↓ 3.0 (± 1.28) |
| Insulin-induced protein 1 | Expressed in proliferating liver during regeneration and development. | ↑ 2.5 (± 0.11) |
| Interferon-inducible protein 1-8U | Laminin receptor homolog | ↓ 2.3 (± 0.18) |
Total RNA derived from KB-D and KB-R cells was labeled with Cy3 or Cy5 fluorescent nucleotide analogs and then hybridized to Human Oncochip cDNA array (National Cancer Institute, Bethesda, MD), as described under Materials and Methods. Results are a compilation of four independent experiments: duplicates of Cy3-labeled KB-R and Cy5-labeled KB-D cDNA, and their reciprocal duplicates. Arrows indicate the detected change in mRNA levels in KB-D cells relative to KB-R cells.