Table 1

Induction of midazolam hydroxylation activity by 1,25-D3 in Caco-2 cells

1,25-D3Dose1-a1′-OH-MDZ Formation Rate1-b
nM pmol/h/insert
0Bld
5018.2  ± 2.01
10032.1  ± 3.56
25041.5  ± 11.0
50036.1  ± 3.83
100038.8  ± 11.6
  • 1-a  Dose concentration applied to the apical compartment every other day for 14 days.

  • 1-b  Product formation rate (mean ± S.D. for triplicate incubations) following application of a single 2 μM dose of midazolam to the apical compartment.

  • Bld, below limit of detection.