IP Antibody | IB Antibody | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
1.1 | 1.2 | 1.3 | 1.4 | 1.5 | 1.6 | |||||||
60kD | 84kD | 65kD | 80kD | 64kD | 88kD | 88kD | 110kD | 62kD | 85kD | 60kD | 88kD | |
1.1 | + | ++ | +++ | ++ | + | ++ | + | − | − | |||
1.2 | ++ | + | ++ | ++ | +++ | ++ | ++ | + | − | |||
1.3 | +++ | + | − | − | + | − | +++ | − | − | |||
1.4 | + | − | − | ++ | − | − | ++ | |||||
1.5 | + | ++ | + | ++ | + | ++ | ++ | ++ | + | |||
1.6 | + | − | ++ | +++ | + | + |
Interactions between different Kv α-subunits as demonstrated by co-immunoprecipitation. Where low- and high-molecular-mass forms of Kv protein were detected, the relative amounts that coassociate are indicated. Minus sign signifies undetectable by immunoblotting. Plus sign indicates apparent relative abundance (+ < ++ < +++). Each cell represents data obtained by immunoprecipitation (IP) and immunoblotting (IB) of multiple membrane preparations from fresh granulosa cells (n = 2–5). Typically, membranes were prepared using granulosa cells harvested from the small and medium follicles on 150 to 300 pig ovaries.