Interactions between different Kv α-subunits as demonstrated by co-immunoprecipitation. Where low- and high-molecular-mass forms of Kv protein were detected, the relative amounts that coassociate are indicated. Minus sign signifies undetectable by immunoblotting. Plus sign indicates apparent relative abundance (⁺ < ⁺⁺ < ⁺⁺⁺). Each cell represents data obtained by immunoprecipitation (IP) and immunoblotting (IB) of multiple membrane preparations from fresh granulosa cells (n = 2–5). Typically, membranes were prepared using granulosa cells harvested from the small and medium follicles on 150 to 300 pig ovaries.