Kd and B max values of [3H]diprenorphine binding to the wild-type, chimeric, and mutant κ opioid receptors stably expressed in CHO cells
| Receptor Construct | Kd | Bmax | n |
|---|---|---|---|
| nM | pmol/mg of protein | ||
| hkor | 0.16 ± 0.02 | 1.22 ± 0.05 | 3 |
| rkor | 0.20 ± 0.20 | 1.00 ± 0.14 | 3 |
| Flag-hkor | 0.10 ± 0.02 | 1.33 ± 0.15 | 6 |
| Flag-rkor | 0.26 ± 0.10 | 2.09 ± 0.31 | 6 |
| Flag-r/hkor | 0.12 ± 0.01 | 2.74 ± 0.14 | 4 |
| Flag-h/rkor | 0.10 ± 0.01 | 2.34 ± 0.13 | 3 |
| Flag-rkorN358S | 0.13 ± 0.02 | 2.25 ± 0.03 | 3 |
| Flag-hkorS358N | 0.08 ± 0.01 | 1.27 ± 0.15 | 6 |
Membranes were prepared from CHO cells stably expressing each receptor construct. Saturation binding of [3H]diprenorphine was performed and K d and B max values were determined. Results are expressed as mean ± S.E.M.n = number of independent experiments in duplicate.