Kinetic analysis of HIV-1 and FIV wild-type and chimeric RT enzymes with poly(rC) · oligo(dG)3-a as the variable template/primer
| RT | Ki,nev | Km | kcat | kcat/Km |
|---|---|---|---|---|
| μM | pmol/μg protein/s | |||
| HIVp66/HIVp51 | 0.55 | 2.9 ± 0.5 | 3.4 ± 0.7 | 1.2 |
| HIVp66/FIVp51 | 1.2 | 10 ± 1.9 | 0.05 ± 0.02 | 0.005 |
| FIVp66/FIVp51 | >50 | 8.8 ± 1.7 | 0.76 ± 0.03 | 0.086 |
| FIVp66/HIVp51 | >50 | 4.2 ± 0.4 | 0.06 ± 0.002 | 0.015 |
The data are means of at least two to three independent experiments (means ± S.D.).
↵3-a dGTP, 1 μCi.