Table 2

Primers used for site-directed mutagenesis of CYP2J2

MutationSequences of Primers
Thr143Ala 5′-AGGTTCACTCTGGCAGCACTAAGG-3′
Arg158Cys 5′-GCTTAGAGGAATGCATTCAGGAGG-3′
Ile192Asn 5′-CCAATATCAATTGCTCCATCACC-3′
Asp342Asn 5′-AGCTGAGATTAACAGAGTGATTGG-3′
Asn404Tyr 5′-GATCCTGACCTATTTGACGGCGC-3′

The underlined bases indicate single nucleotides changes.