Sensitivity of berberrubine-resistant cells to various antitumor drugs
| AMC5/B1 | |||
|---|---|---|---|
| Drug | AMC5 (IC50) | IC50 | R |
| μM | μM | ||
| Berberrubine | 35.1 ± 14.3 | 187.5 ± 17.7 | 5.3 |
| Etoposide | 54.2 ± 6.0 | 293.6 ± 42.9 | 5.4 |
| Doxorubicin | 2.2 ± 1.8 | 10.5 ± 0.3 | 4.8 |
| Mitoxantrone | 0.8 ± 0.09 | 2.1 ± 0.04 | 2.6 |
| m-AMSA | 8.6 ± 2.6 | 16.5 ± 0.9 | 1.9 |
| Ellipticine | 0.9 ± 0.05 | 1.7 ± 0.63 | 1.9 |
| Camptothecin | 1.0 ± 0.04 | 1.1 ± 0.19 | 1.1 |
| Cisplatin | 41.9 ± 4.7 | 54.3 ± 6.9 | 1.3 |
| Vinblastine | 0.5 ± 0.02 | 0.5 ± 0.02 | 1.0 |
IC50 values were measured using the MTT assay. Values are means ± S.D. of three or more determinations. Resistance factor (R) was calculated as the ratio of IC50 values between AMC5/B1 and AMC5 cells.
m-AMSA, 4′-(9-acridinyl-amino)methanesulfon-m-anisidide (amsacrine).