Table 3

Binding properties of hCRF2A, xCRF2, and the receptor mutants hR2KYI and xR2DLV using 125I-astressin as radioligand

PeptidehCRF2AhR2KYIxR2DLVxCRF2
nM
hCRF125  ± 193-a 57.7  ± 8.23-a 166  ± 173-a 69.8  ± 5.13-a
oCRF949  ± 1083-b 416  ± 113-b 1020  ± 583-b 604  ± 803-b
hUCN0.79  ± 0.051.36  ± 0.191.14  ± 0.090.29  ± 0.06
Sauvagine26.6  ± 2.920.6  ± 1.824.7  ± 3.114.5  ± 2.3
Astressin1.26  ± 0.041.33  ± 0.090.99  ± 0.070.89  ± 0.15
aSVG1.94  ± 0.2417.4  ± 0.83-c 1.37  ± 0.1524.6  ± 3.33-c

The data are mean K i values ± S.E.M. from at least three different binding experiments performed in triplicate using 125I-sauvagine as radioligand.

    • ↵3-a  p < 0.0001 vs. hUCN, sauvagine, astressin, and aSVG.

    • ↵3-b  p < 0.0001 vs. hCRF, hUCN, sauvagine, astressin, and aSVG.

    • ↵3-c  p < 0.005 vs. hCRF2A and xR2DLV.