Yeast strains used in our studies
| Strain | Genotype |
|---|---|
| RHY718 | MATα ade2–101 met2 his3Δ200 hmg2::HIS3 lys2–801 hmg1::LYS2 leu2Δ trp1Δ ura3–52 + pRH244 (URA3,6mycHMG2) |
| RHY609 | MATα ade2–101 met2 his3Δ200 hmg2::HIS3 lys2–801 hmg1::LYS2 LEU trp1::hisG ura3–52::6MycHMG2 hrd1::URA3 |
| RHY925 | MATα ade2–101 met2 his3Δ200 hmg2::HIS3 lys2–801 hmg1::LYS2 leu2Δ trp1Δ URA3::6MycHMG2 hrd2–1 |
| RHY749 | MATα ade2–101 met2 his3Δ200 hmg2::HIS3 lys2–801 hmg1::LYS2 leu2Δ trp1Δ ura3–52::6MycHMG2 hrd3::URA3 |
| RHY1166 | MATα ade2–101 met2 his3Δ200 hmg2::HIS3 lys2–801 hmg1::LYS2 leu2Δ trp1Δ ura3–52::MycHMG2 |
| RHY1596 | MATα ade2–101 met2 his3Δ200 hmg2::HIS3 lys2–801 hmg1::LYS2 leu2Δ trp1Δ ura3–52::MycHMG2 ubc6::KanMX |
| RHY1603 | MATα ade2–101 met2 his3Δ200 hmg2Δ-4 lys2–801 hmg1::LYS2 leu2Δ trp1Δ ura3–52::MycHMG2 ubc7::HIS3 |
| RHY1604 | MATα ade2–101 met2 his3Δ200 hmg2Δ-4 lys2–801 hmg1::LYS2 leu2Δ trp1Δ ura3–52::MycHMG2 ubc6::KanMX ubc7::HIS3 |
| RHY473 | ade2–101 met2 his3Δ200 lys2–801 ura3–52 |
| RHY106-4 | ade2–101 met2 his3Δ200 lys2–801 ura3–52 pep4Δ |
Strains RHY718, -609, -925, and -749 are an isogenic set and correspond to wt and hrd1-, hrd2-, and hrd3-deficient S. cerevisiae mutants. RHY1166, -1596, -1603 and 1–604 are also an isogenic set and correspond to wt and ubc6-,ubc7-, and ubc6/7-deficient S. cerevisiae mutants. Strains RHY473 and -106-4 correspond toPEP4 and pep4Δ. The method of their construction has been described previously (Hampton and Rine, 1994;Hampton et al., 1996; Wilhovsky et al., 2000).