Effects of halothane on retinal binding kinetics
| Halothane | 0.0 mM | 1.0 mM | 2.0 mM | 4.0 mM | 6.0 mM |
|---|---|---|---|---|---|
| Absorbance Increase Half-Time | |||||
| T1 (s) | 47 ± 6 | 53 ± 2 | 63 ± 5 | 76 ± 71-150 1-151 | |
| T2 (min) | 17 ± 2 | 23 ± 4 | 25 ± 2 | 35 ± 61-150 | |
| Fast component (%) | 68 ± 3 | 57 ± 71-150 | 50 ± 31-150 | 38 ± 21-150 1-151 | |
| Fluorescence Decrease Half-Time | |||||
| T1 (s) | 8.0 ± 0.6 | 9.3 ± 0.5 | 12.4 ± 1.21-150 1-152 | 13.6 ± 1.01-150 1-152 | |
| T2 (min) | 3.4 ± 0.3 | 5.3 ± 0.5 | 6.8 ± 0.91-150 | 12.2 ± 1.11-150 1-152 | |
Half-times of retinal binding kinetics monitored by the increase in the absorbance and the fluorescence decrease. For the increase in the absorbance, the data are fitted to a two-phase exponential association. For the fluorescence decrease, the data were fitted to a two-phase exponential decay. Values are means ± S.E.M. (n = 5 for absorbance, n = 4 for fluorescence). All fits showed an R 2value > 0.99.
↵1-150 P < 0.05 compared with the absence of halothane [one-way analysis of variance (ANOVA) followed by Student-Neuman-Keuls test].
↵1-151 P < 0.05 compared with halothane 1.0 mM (one-way ANOVA followed by Student-Neuman-Keuls test).
↵1-152 P < 0.05 compared with halothane 2.0 mM (one-way ANOVA followed by Student-Neuman-Keuls test).