cAMP response for the single proline-to-alanine mutations
| Iloprost | EC50 MUT / WT | |
|---|---|---|
| nM | ||
| hIP1D4PMT4 (WT) | 1.2 ± 0.3 | 1 |
| P17A (I) | 6.3 ± 1.3 | 5 |
| P69A (II) | 25.7 ± 5.4 | 21 |
| P89A (III) | 12.0 ± 2.5 | 10 |
| P141A (IV) | 1.1 ± 0.3 | 1 |
| P154A (IV) | 28.0 ± 18.0 | 23 |
| P179A (V) | 0.9 ± 0.1 | 1 |
| P254A (VI) | 8.2 ± 3.0 | 7 |
| P285A (VII) | 0.9 ± 0.3 | 1 |
| P289A (VII) | 6.3 ± 2.2 | 5 |
EC50 values (mean ± S.E.) for single proline-to-alanine mutations from at least three separate experiments (duplicates of six different concentrations). Bold numbering indicates significant decrease in EC50 compared with wild-type (hIP1D4PMT4).