Iloprost | EC50 MUT / WT | |
---|---|---|
nM | ||
hIP1D4PMT4 (WT) | 1.2 ± 0.3 | 1 |
P17A/P179A | 9.4 ± 0.7 | 8 |
P69A/P89A | 10.5 ± 1.5 | 9 |
P141A/P285A | 0.6 ± 0.3 | 1 |
P17A/P69A/P89A | 2824 ± 2167 | 2353 |
P141A/P179A/P285A | 57 ± 46 | 48 |
EC50 values (mean ± S.E.) for combination proline-to-alanine mutations. Bold numbering indicates significant decrease in EC50 compared with wild-type (hIP1D4PMT4).