Table 1

Efficiency of detergents to solubilize P-gp from plasma membranes of CCRF-ADR5000 cells

DetergentSolubilization Efficiency 1-a
%
n-Octylglucoside12
Triton X-10033
Nonidet P-4092
Deoxy-BIGCHAP6
Deoxycholate47
N-Lauroylsarcosine103
Zwittergent 3-1217
CHAPS3

Membrane proteins were labeled with 1.6 μM [3H]azidopine, homogenized in the presence of 1% detergent and incubated for 30 min at 4°C. The nonsolubilized membrane proteins were separated by ultracentrifugation, and the supernatant was analyzed by SDS-PAGE. The solubilization efficiency was determined by liquid scintillation counting of the excised radioactive P-gp band. Solubilization of efficiency is given relative to its solubilization with PAGE-sample buffer containing 2% SDS.

    • ↵1-a Standard deviation of the P-gp solubilization efficiency was approximately 5%.