Cell Line | Covalent Binding | IC50 |
---|---|---|
pmol bound AF/mg protein | μM | |
MCF7 | 28 | 0.035 |
Caki-1 | 12 | 0.130 |
OVCAR5 | 41 | 0.028 |
MDA-MB-435 | 0.9 | >86 |
M14-MEL | 0.4 | >37 |
HT-29 | 0.2 | >29 |
AF was incubated with cells for 72 h, and IC50 values determined with the MTT assay as described under Materials and Methods. Covalent binding was determined following 12-h exposure of 50 nM radiolabeled AF and isolation of cellular macromolecules by acetone precipitation followed by solvent washing (see Materials and Methods). Values are means of at least three experiments.