Table 4

Midazolam hydroxylation phenotype as a function ofCYP3A5 genotype

	All Genotypes	CYP3A5^4-1501/^4-1501	CYP3A5^4-1501/^4-1503	CYP3A5^4-1503/^4-1503
Liver microsomal activity	(n = 60)	(n = 1)	(n = 13)	(n = 36)
1′-Hydroxylation (pmol/min/mg)	880 (174–4842)	4443	1893 (505–4842)	660 (174–3597)^4-160
4-Hydroxylation (pmol/min/mg)	148 (26–850)	502	294 (40–850)	124 (26–699)
1′-/4-Hydroxymidazolam ratio	6.1 (4.8–13.5)	8.9	6.4 (5.7–13.5)	5.4 (4.8–6.8)
Total activity (pmol/min/mg)	1026 (200–5692)	4844	2188 (545–5692)	787 (317–4348)^4-150
Jejunal homogenate activity	(n = 31)	(n = 2)	(n = 2)	(n = 27)
1′-Hydroxylation (pmol/min/mg)	148 (28–348)	295 (278,311)	65.5 (41,90)	148 (28–348)
4-Hydroxylation (pmol/min/mg)	26.1 (2–60.6)	35.9 (29.3,42.5)	5.8 (4.8,6.8)	26.1 (2–60.6)
1′-/4-Hydroxymidazolam ratio	6.8 (4.0–13.1)	8.4 (7.3,9.5)	10.9 (8.6,13.1)	6.3 (4.0–7.9)
Total activity (pmol/min/mg)	173 (30–409)	330 (307,353)	71.0 (46,96)	173 (30–409)

Data are reported as median (range).