Effect of SB-431542 on the activities of protein kinases in vitro
| Protein Kinase | Activity |
|---|---|
| % control | |
| ALK21-a | 120 |
| ALK41-a | 12 |
| ALK61-a | 50 |
| AMPK | 97 |
| Chk1 | 100 |
| CK2 | 93 |
| GSK3β | 116 |
| JNK1 | 99 |
| lck | 104 |
| MAPK2 | 98 |
| MAPKAP-K1β | 113 |
| MAPKAP-K2 | 95 |
| MKK1 | 104 |
| MSK1 | 109 |
| p70 S6K | 100 |
| PDK1 | 92 |
| Phos. kinase | 101 |
| PKA | 111 |
| PKBα | 89 |
| PKCα | 99 |
| PRAK | 80 |
| ROKα | 98 |
| p38 MAPKα | 51 |
| p38 MAPKβ | 90 |
| p38 MAPKγ | 110 |
| p38 MAPKδ | 99 |
| SGK | 92 |
Protein kinases were assayed with 10 μM SB-431542 in the presence of 0.1 mM ATP. Activities are given as the mean of duplicate determinations relative to control incubations with DMSO vehicle.
↵1-a For ALK2, ALK4, and ALK6, purified kinase domains minus the GS regions were used.