Regakine-1, C5a, or both were added to the lower compartment of the microchamber to measure human neutrophil chemotaxis. Human neutrophils were added to the upper compartment of the microchamber, either directly with regakine-1 (no preincubation) or after 10 min of preincubation (at 37° C) and subsequent removal of regakine-1 (by washing twice). C5a or buffer was always added to the lower compartment of the microchamber. The results are expressed as the mean CI ± S.E.M., derived from three (lower wells) or six (upper wells) independent experiments. Statistically significant differences between C5a alone or in combination with regakine-1 were determined by the Mann-Whitney test.

• ↵2-150  p < 0.05; ** p < 0.01.