Regakine-1 fails to synergize with other neutrophil chemoattracting CC chemokines in neutrophil chemotaxis
| Chemokine | Regakine-1 | CI ± S.E.M. |
|---|---|---|
| ng/ml | ||
| Buffer | 30 | 5.0 ± 1.0 |
| Buffer | 100 | 5.5 ± 2.4 |
| MCP-3 (30) | 0 | 2.0 ± 0.7 |
| MCP-3 (30) | 30 | 3.9 ± 1.2 |
| MCP-3 (30) | 100 | 5.3 ± 1.5 |
| MCP-3 (100) | 0 | 2.3 ± 0.4 |
| MCP-3 (100) | 30 | 5.2 ± 1.8 |
| MCP-3 (100) | 100 | 7.3 ± 4.4 |
| MIP-1α/LD78β (30) | 0 | 2.3 ± 0.6 |
| MIP-1α/LD78β (30) | 30 | 4.7 ± 1.1 |
| MIP-1α/LD78β (30) | 100 | 6.1 ± 1.2 |
| MIP-1α/LD78β (100) | 0 | 1.9 ± 0.3 |
| MIP-1α/LD78β (100) | 30 | 2.7 ± 4.3 |
| MIP-1α/LD78β (100) | 100 | 6.8 ± 1.9 |
MCP-3 or MIP-1α/LD78β were combined with different concentrations of regakine-1 in the lower compartment of the microchamber to measure human neutrophil chemotaxis. The chemotactic response is expressed as the mean CI ± S.E.M., derived from four independent experiments.