Predicted molecular masses derived from the primary structure, masses determined by mass spectroscopy, and the K ivalues of the respective proteins for the interaction with thrombin
| Primary Structure | MALDI-MS | Ki | |
|---|---|---|---|
| Da | M +H+ | pM | |
| H6-Sak-Dip-I | 23,167 | 23,264 | 353 |
| Dip-I | 6,083 | 6,077 | 75 |
| H6-Sak-Dip-II | 23,717 | 23,750 | ≫353 |
| Dip-II | 6,598 | 6,576 | ≫353 |
| H6-Sak-Dip-I +II | 29,096 | 29,158 | 0.91 |
| Dip-I +II | 12,011 | 11,995 | 0.78 |
| H6-Sak | 17,102 | 17,175 | ≫353 |
| Sak42D | 15,583 | N.D. | N.D. |
N.D., not determined; MALDI-MS, matrix-assisted laser desorption ionization-mass spectrometry.