Effect on CLC Channels Expressed in Oocytes | ||||||
---|---|---|---|---|---|---|
Compound | Effect on Muscle gCl | CLC-1 | D9 | ΔNCLC-2 | CLC-5 | CLC-Kb(c) |
% reduction | ||||||
CPA (N1) | – | – | – | – | N.D. | |
CPP (N2) | 58 ± 131-150 | – | N.D. | – | – | N.D. |
CPV (N3) | – | N.D. | – | – | N.D. | |
CPPA (N4) | – | – | – | – | N.D. | |
N5 | 16 ± 61-150 | – | – | – | – | N.D. |
N6 | 4 ± 6 | N.D. | N.D. | N.D. | – | N.D. |
N7 | 57 ± 101-150 | N.D. | N.D. | N.D. | – | N.D. |
N8 | 15 ± 61-150 | N.D. | N.D. | N.D. | N.D. | N.D. |
N9 | 2 ± 7 | N.D. | – | N.D. | N.D. | N.D. |
N10 | 0 ± 7 | N.D. | N.D. | N.D. | N.D. | N.D. |
N11 | 13 ± 71-150 | – | 1-213 | – | – | N.D. |
N12 | 25 ± 81-150 | 1-213 | N.D. | N.D. | N.D. | |
N13 | 46 ± 101-150 | – | 1-213 | – | – | 1-213 |
N14 | 33 ± 81-150 | – | 1-213 | N.D. | – | N.D. |
N15 | 32 ± 81-150 | – | 1-213 | N.D. | – | N.D. |
N16 | 48 ± 91-150 | – | – | N.D. | N.D. | N.D. |
For the evaluation on heterologously expressed channels currents were measured using two-electrode voltage clamp as described under Materials and Methods; substances were applied at 200 μM. At least three oocytes were measured for each tested substance. For CLC-1 and D9, only the “short-term” effects are evaluated in the Table (i.e. effects after a brief incubation of <30 s). After a longer incubation time, significant effects were observed for CLC-1 for the strict derivatives of CPP (compounds N2 to N4) (Pusch et al., 2000). For CLC-5 and Δ NCLC-2, the effects were evaluated after about 5 to 10 min of incubation.