Table 1

Cer and gangliosides levels after PDMP treatment of KG1a and KG1a/200 cells

	Cer	Glu-Cer	Lac-Cer	GM3	GD3
	%
KG1a
Control	100 ± 7	100 ± 6	100 ± 7	100 ± 10	100 ± 3
PDMP 24 h	124 ± 5^1-150	37 ± 2^1-150	46 ± 8^1-150	80 ± 10^1-150	132 ± 35
PDMP 48 h	129 ± 6^1-150	N.T.	50 ± 23^1-150	82 ± 8^1-150	92 ± 20
PDMP 72 h	188 ± 6^1-150	N.T.	52 ± 23^1-150	67 ± 9^1-150	116 ± 27
KG1a/200
Control	100 ± 7	100 ± 6	100 ± 4	100 ± 5	100 ± 4
PDMP 24 h	183 ± 18^1-150	32 ± 2^1-150	56 ± 22^1-150	74 ± 15^1-150	51 ± 17^1-150
PDMP 48 h	176 ± 9^1-150	N.T.	55 ± 27^1-150	55 ± 11^1-150	67 ± 18^1-150
PDMP 72 h	317 ± 45^1-150	N.T.	45 ± 10^1-150	59 ± 20^1-150	50 ± 2^1-150

KG1a and KG1a/200 cells were labeled with [³H] palmitic acid for 48 h and treated for various times with 20 μM PDMP. The levels of Cer and gangliosides were analyzed as described underExperimental Procedures. Results are calculated as percentages compared with untreated cells and are mean ± S.D. of at least three independent experiments. Statistical analyses were performed using the Student's t test.

N.T., not tested.
↵1-150 Significant differences (P < 0.05) compared with untreated cells.