Mean closed times of mutant nAChRs activated by 30 μM ACh
| Number of F Residues | Mutant Subunits | Mean Closed Time | n |
|---|---|---|---|
| ms | |||
| 0 | αF8′I | 1.80 ± 0.60 | 4 |
| 1 | αF8′I, δL8′F | 2.30 ± 0.25 | 3 |
| 2 | Wild-type | 1.30 ± 0.18 | 6 |
| 3 | ɛV8′F | 2.40 ± 0.14 | 4 |
| 4 | ɛV8′F, δL8′F | 2.70 ± 0.20 | 5 |
| 4 | βL8′F, δL8′F | 5.60 ± 1.70 | 4 |
| 5 | βL8′F, ɛV8′F, δL8′F | 5.00 ± 1.20 | 5 |
Recordings were obtained from HEK cells transfected with wild-type and mutant subunits to yield nAChRs containing different number of phenylalanine residues at 8′, ranging from 0 (F = 0) to 5 (F = 5). The mean closed time corresponds to the predominant component of the closed-time histogram which is associated to closings within clusters. n is the number of recordings for each condition.