Table 1

Primers used for PCR

Primer	Orientation	Oligonucleotide Sequence (5′-3′)	nt Assignment
P1	Sense	`T7-AGGCTCTGACAGCCCAGAGTT`	3618–3638
P2^1-a	Antisense	`CGAATTGGAGCTCCACCGC`	11–29
P3	Sense	`T7-GTGTCTGCAGCACTTGGATC`	2998–3017
P4	Antisense	`ACTCAGTGCCAGAAGCTGGA`	3638–3657
P5	Sense	`T7-ACAGCCCAGAGTTCCAGCT`	3626–3644
P6	Antisense	`GGAGAGAGATTTAGTAGTCCAC`	3762–3783
P7	Sense	`T7-GTGGACTACTAAATCTCTCT`	3762–3781
P8	Antisense	`CTGTACATAGTGCAGCTAA`	3961–3979
P9	Sense	`T7-ATACTTAGCTGCACTATGTACAG`	3957–3979
P10	Sense	`T7-TTGGAGCTGAGAGCAGAG`	3874–3891
P11	Antisense	`CTGTACATAGTGCAGCTAAG`	3960–3979
P12	Antisense	`ATTGGGAGTAGACAAAAGTATCT`	4007–4029
P13	Sense	`T7-GATGGCTTGGGCCTTTCCT`	4030–4048
P14	Sense	`T7-AATATTTATATAAAATACA`	4068–4086

The different sets of primers used to amplify various fragments from iNOS coding sequence and 3′UTR are shown, as seen in Figure 2. T7 represents the T7 RNA polymerase promoter sequence 5′-TAATACGACTCACTATAGGGAGA-3′. The nucleotide assignments correspond to the iNOS sequence in GenBank reference number M92649 (Lowenstein et al, 1992).

P, Primer; nt, nucleotide.
↵1-a P2 is within the pBluescript multiple cloning site flanking the cloned iNOS cDNA (in the NotI site). All fragments produced with P2 were digested withNotI to remove the additional 29 nt.