Cell Lines | IC50 | |
---|---|---|
As2O3 | Paclitaxel | |
μM | ||
MCF-7 | 3.00 ± 0.20 | 0.66 ± 0.33 |
MCF-7/Dox (P-gp positive) | 7.00 ± 1.70 | 23.30 ± 10.69 |
Resistance index | 2.3 | 35 |
MCF-7/VP-16 (MRP positive) | 5.56 ± 2.11 | 2.57 ± 1.36 |
Resistance index | 1.9 | 4 |
A2780 | 2.80 ± 0.70 | 0.04 ± 0.08 |
A2780/PTX10 (tubulin mutant) | 2.41 ± 0.92 | 0.59 ± 0.16 |
Resistance index | 0.86 | 15 |
A2780/PTX22 (tubulin mutant) | 2.61 ± 1.36 | 0.66 ± 0.13 |
Resistance index | 0.93 | 17 |
Cells were treated with different concentration of drugs at 37° C for 72 h. Cell survival was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium assay, and IC50 was calculated as described under Materials and Methods. Each value represents the mean ± S.D. of three independent experiments. Resistance index = IC50 in resistant cells/IC50 in parental cells.
P-gp, P-glycoprotein.