Primers used for directed mutagenesis
| Primers | Sequences | |
|---|---|---|
| UGT 1A1 | ||
| *27 | A | 5′-cgtggtttattcccagtatgcaacccttg-3′ |
| B | 5′-caagggttgcatactgggaataaaccacg-3′ | |
| *7 | C | 5′-ctcacctggtaccaggaccattccttggacg-3′ |
| D | 5′-cgtccaaggaatggtcctggtaccaggtgag-3′ | |
| *6 | E | 5′-cgcctcgttgtacatcagagacagagcattttacaccttg-3′ |
| F | 5′-caaggtgtaaaatgctctgtctctgatgtacaacgaggcg-3′ | |
| *35 | G | 5′-ttattccccgtatgcaacccttgcctcagaattccttcaga-3′ |
| H | 5′-tctgaaggaattctgaggcaagggttgcatacggggaataa-3′ | |
| UGT 1A7 | ||
| *2 | I | 5′-caaggagagagtatggaaccacatcatgcac-3′ |
| J | 5′-gtgcatgatgtggttccatactctctccttg-3′ | |
| *4 | K | 5′-ggagtttgtttaatgaccgaaaattagtag-3′ |
| L | 5′-ctactaattttcggtcattaaacaaactcc-3′ |
Bold letters represent mutations produced by PCR-based mutagenesis