Summary of MEK/p38-dependent or -independent Akt S473 phosphorylation in cells
| Cell Type | Cell Line | Change in Akt S473 Phosphorylation | Inhibition by PD98059 | Inhibition by SB203580 | |||
|---|---|---|---|---|---|---|---|
| LPA | S1P | LPA | S1P | LPA | S1P | ||
| -fold | % | % | |||||
| Ovarian Cancer | HEY | 8.2 ± 0.9 | 10.1 ± 1.2 | 94 ± 5 | 91 ± 6 | 99 ± 1 | 99 ± 1 |
| Ovca420 | 3.9 ± 1.3 | 3.6 ± 1.4 | 78 ± 10 | 79 ± 8 | 83 ± 10 | 88 ± 8 | |
| Ovca429 | 2.3 ± 0.7 | 2.4 ± 0.5 | 83 ± 5 | 92 ± 4 | 99 ± 2 | 99 ± 1 | |
| Ovca432 | 3.9 ± 1.1 | 4.4 ± 0.8 | 80 ± 8 | 88 ± 3 | 80 ± 9 | 94 ± 8 | |
| Ovca433 | 2.3 ± 0.3 | 2.8 ± 0.7 | 77 ± 5 | 86 ± 4 | 92 ± 5 | 97 ± 4 | |
| A2780 | 2.6 ± 0.2 | 3.7 ± 0.4 | 78 ± 5 | 84 ± 6 | 85 ± 6 | 92 ± 3 | |
| Other | HeLa | 5.1 ± 1.4 | 6.4 ± 2.1 | 89 ± 7 | 86 ± 10 | 97 ± 2 | 99 ± 1 |
| MCF10A | 1.1 ± 0.2 | 0.9 ± 0.1 | N.A. | N.A. | N.A. | N.A. | |
| MDA-MB-231 | 3.3 ± 1.5 | 4.0 ± 1.1 | 20 ± 5 | 27 ± 6 | 40 ± 3 | 39 ± 5 | |
| T-47D | 5.2 ± 0.7 | 4.3 ± 0.9 | 92 ± 5 | 90 ± 3 | 97 ± 2 | 95 ± 4 | |
| GI-101A | 6.8 ± 2.4 | 5.8 ± 1.2 | 0 ± 10 | 0 ± 10 | 80 ± 10 | 96 ± 3 | |
| PC-3 | 1.7 ± 0.3 | 1.7 ± 0.2 | 0 ± 10 | 0 ± 10 | 96 ± 8 | 93 ± 12 | |
Cells were pretreated with PD98059 (30 μΜ) or SB203580 (10 μM) for 30 min, then treated with LPA (10 μM) or S1P (1 μM) for 20 min. Western analyses of cell lysates were probed with phospho-specific Akt S473 or Akt antibodies. Fold-change in Akt phosphorylation is normalized to the levels of total Akt.
N.A., not applicable.