2-Δ Δ CT | ||||||||
---|---|---|---|---|---|---|---|---|
S1P Receptors | LPA Receptors | |||||||
Cell Line | S1P1 | S1P2 | S1P3 | S1P5 | LPA1 | LPA2 | LPA3 | |
HEY | 4.2 | 1.1 | 3.0 | 0.3 | 2.2 | 1.0 | 7.6 | |
Ovca420 | 0.3 | 10.4 | 4.6 | 14.5 | 172.4 | 21.4 | 0.2 | |
Ovca429 | 3.7 | 11.6 | 26.4 | 0.4 | 12.6 | 4.9 | 1.9 | |
Ovca432 | 1.1 | 8.3 | 46.9 | 0.2 | 15.9 | 6.1 | 13.0 | |
Ovca433 | 15.6 | 7.5 | 1.6 | 0.3 | 6.0 | 2.3 | 1.8 | |
A2780 | 0.8 | 18.5 | 21.6 | 0.6 | 6.2 | 3.9 | 7.6 | |
HeLa | 0.0 | 4.6 | 6.0 | 0.4 | 0.0 | 1.4 | 2.8 | |
MCF10A | 0.2 | 1.6 | 1.5 | 0.1 | 0.7 | 10.7 | 0.8 | |
MDA-MB-231 | 0.1 | 4.3 | 2.1 | 0.1 | 83.9 | 5.8 | 1.9 | |
T-47D | 0.0 | 7.8 | 11.4 | 7.9 | 37.3 | 28.4 | 0.1 | |
GI-101A | 0.0 | 10.3 | 0.3 | 0.4 | 0.1 | 11.5 | 15.7 | |
PC-3 | 0.1 | 1.9 | 0.1 | 0.0 | 37.5 | 2.9 | 29.9 |
The relative expression of LPA2 in HEY cells was chosen as 1-fold. The relative amounts of other receptors are expressed as a fold change compared with LPA2 in HEY cells.
CT = threshold cycle; Δ CT = CT for target gene − CT for GAPDH gene; Δ Δ CT = Δ CT relative to HEY LPA2 (Δ CT for target gene in test cell line − Δ CT for LPA2 in HEY cells); 2-Δ Δ CT = measurement of expression of target gene relative to LPA2in HEY cells.