Specific activity of Cdk2 kinase.
| Immunoprecipitated | ||
|---|---|---|
| Cdk 2 | Cyclin A | |
| S-enriched fraction | 356 ± 4.5 | 352 ± 12.3 |
| 1 μM F-ara-A | ||
| 24 h | 199 ± 5.1 | 275 ± 6.4 |
| 27 h | 327 ± 7.8 | 272 ± 7.1 |
| 30 h | 272 ± 7.6 | 315 ± 12.3 |
| 1 μM F-ara-A, 27 h | ||
| + 50 nM UCN-01, 3 h | 381 ± 16.4 | 543 ± 10.1 |
| 1 μM F-ara-A, 30 h | ||
| + 50 nM UCN-01, 6 h | 639 ± 2 | 697 ± 77 |
Values are expressed as counts per minute normalized to Western blot densitometric units.