Phosphorylation of [8-3H]GCV in rVV-infected human 143B cells
| Phosphorylated metabolites of [8-3H]GCV | ||||
|---|---|---|---|---|
| MP | DP | TP | Total | |
| pmol/107 cells | ||||
| rVV-GS | 1.1 ± 0.53-150 | 1.7 ± 0.333-150 | 4.6 ± 4.5 | 7.4 ± 4.33-150 |
| rVV-Z29 | 1.7 ± 1.23-150 | 1.5 ± 0.163-150 | 4.0 ± 3.7 | 7.1 ± 4.83-150 |
| rVV-UL97 | 24 ± 22** | 9.7 ± 3.1** | 27 ± 20** | 61 ± 41** |
| wtVV | 0.44 ± 0.47 | 0.54 ± 0.32 | 2.0 ± 1.5 | 3.0 ± 2.0 |
| Mock-infected | 0.19 ± 0.21 | 0.47 ± 0.21 | 1.2 ± 0.70 | 1.8 ± 1.0 |
Two hours postinfection, cells were pulsed with [8-3H]GCV (5 μ M) for 24 h. Phosphorylated metabolites were measured by anion-exchange HPLC and liquid scintillation counting. Values are means ± S.D. for three independent experiments.
MP, monophosphate metabolites; DP, diphosphate metabolites; TP, triphosphate metabolites.
↵3-150 P < 0.05; **P < 0.01; statistical significance compared with mock-infected condition (Wilcoxon two-sample test).