Displacement of LPS-bound dansyl-PMBN by PMBN analogs. Increasing concentrations of PMBN peptides were added to E. coli- LPS solution (3 μg/ml) bound to dansyl-PMBN (0.55 μM). The fluorescence inhibition was measured 5 min after each addition at excitation and emission wavelengths of 340 and 485 nm, respectively. Data represent the mean derived from four experiments. Analytical HPLC was preformed on VydacTM RP-18 column using a linear gradient of t = 0 min, B = 0%; t = 55 min, B = 80%. Grand average of hydropathicity (GRAVY) was calculated using the Web-available program developed by Kyte and Doolittle (http://www.expasy.ch/cgi-bin/protscale.pl).