Table 1

Specificity-control and neutralizing capacity of the monoclonal anti-rat-TNF-α antibodies

	Nitrite
		⁺anti-TNF-α
	μM
Resting	0.3 ± 0.3	0.5 ± 0.3
IL-1β	2.2 ± 0.6	2.8 ± 1.0
TNF-α	0.5 ± 0.3	0.7 ± 0.2
IL-1β ⁺ TNF-α	5.5 ± 0.6^1-159	2.6 ± 0.4^1-150

Resting rat aorta endothelial cells (1 × 10⁵) were incubated for 24 hours with IL-1β (200 U/ml) and/or rat TNF-α (500 U/ml) or alternatively activated in the presence of 150 μg/ml of the monoclonal anti-rat-TNF-α antibody. Then, nitrite concentrations were determined in culture supernatants using the Griess reaction. Values represent the mean ± S.D. of three individual experiments.

↵1-150 , p < 0.001 compared with respective cytokine activated cell cultures grown in the absence of the anti-rat-TNF-α antibody.
↵1-159 , p < 0.001 compared with IL-1β only activated cell cultures.