Cell Line | Vincristine | Daunomycin | Dexamethasone | Puromycin | Colchicine | Mutation |
---|---|---|---|---|---|---|
MSPD-10 | N.C. | ▾ | ▾▾▾ | ▾▾ | ▾▾ | W231C |
MSPT-7 | ▾▾ | ▾▾▾ | ▾▾▾ | ▾▾▾ | ▾ | A229V, W231L |
MSPD-60 | ▾▾ | N.C. | ▾▾▾ | ▾▾ | ▾▾ | A301D |
MSMP-1 | ▾ | ▾▾ | ▾▾ | ▾▾ | ▾▾▾ | S308L |
MSAC-52 | ▾ | N.C. | ▾▾ | N.C. | +++ + | L338F |
The effects of the listed drugs on cell growth was determined for the W7TB, MS23, and variant cell lines as described under Materials and Methods. These measurements allowed the evaluation of an IC50 value that reflects the reduction in cell growth by 50%. The change in IC50 values between a variant line and MS23 is normalized to the difference in IC50 values between W7TB and MS23. The percentage of normalized change in resistance (N.C.R) is defined by the equation: N.C.R = 100% × (VARIC50 − MS23IC50)/(MS23IC50 −W7TBIC50)
N.C., no change; ++++, NCR increase ≥200%; ▾, NCR decrease ≥33%; ▾▾, ≥67%; ▾▾▾, ≥100%.