H2122 cells were stably transfected with retroviruses encoding full-length PPARγ and individual clones were selected for resistance to G-418. Control cells (Neo) were transfected with a retroviral vector lacking an insert. Three clones overexpressing PPARγ and one Neo clone were transiently transfected with the PPAR-RE promoter construct as described in Fig. 2. Cells were stimulated for 24 h with either 10 μ M ciglitazone or 25 μ M sulindac sulfide, and promoter activity normalized to β -gal was determined. Results represent the mean of three independent experiments.