Equilibrium binding constants determined by SPR
| DNA | Keq | |||
|---|---|---|---|---|
| AT2433-A1 | AT2433-B1 | iso-AT2433-B1 | JDC-277 | |
| M−1 | ||||
| [AT]4 | 6.7 105(2) | 1.3 106(4–5) | 1.4 105(6–8) | 1.0 105 (4) |
| [CG]4 | 6.6 106 (3) | 8.9 106(3) | 5.0 105 (4–6) | 5.0 105 (4) |
Experiments were performed in HBS-EP buffer at 25°C. The DNA sequences show one strand of the duplex stem of the hairpin used in the BIAcore SPR experiments. In each case, the number of compound binding sites on the DNA duplex is indicated (n).