Gα13 C-terminal blocking peptide, but not Gαq peptide, prevents PLD activation
| Treatment | PLD Activity | PLC Activity |
|---|---|---|
| -fold basal | ||
| Basal | 1.00 ± 0.13 | 1.00 ± 0.05 |
| Control (5-HT alone) | 3.69 ± 0.70 | 2.67 ± 0.28 |
| G13CT | 1.31 ± 0.091-150 | 2.20 ± 0.42 |
| Gq blocking | 3.94 ± 1.08 | 0.52 ± 0.151-150 |
| GsCT | 3.49 ± 0.44 | 2.52 ± 0.57 |
PLD and PLC activity were measured as described under Materials and Methods. 5-HT (30 nM) was added with and without pretreatment with 100 μM G13CT or PLCβ 1MC or with 50 μM GsCT.
↵1-150 , P < 0.05 compared with control (5-HT alone);n = 3.