Primer pairs
| Receptor | Primer | Amplification Fragment |
|---|---|---|
| P2Y1 | ||
| Forward | 5′-TGGCGTGGTGTACCCTCTCAAGTC-3′ | 410 bp |
| Reverse | 5′-ACCGTGCTCGCAAATTCATCGTT-3′ | |
| P2Y2 | ||
| Forward | 5′-ACCAGCGTGCGGGGAACC-3′ | 440 bp |
| Reverse | 5′-GCATCTCGGGCAAAGCGGACAAGT-3′ | |
| P2Y4 | ||
| Forward | 5′-TGCCTCGTGCCCAACCTCTTCTTT-3′ | 499 bp |
| Reverse | 5′-CAGTTGTTCGGCGCTTAGGTGTGC-3′ | |
| P2Y6 | ||
| Forward | 5′-CCTGGCACTGGCGGACCTGAT-3′ | 452 bp |
| Reverse | 5′-GGCGGGCCATGCGACAATAAC-3′ | |
| β-actin (control) | ||
| Forward | 5′-ATCCATGAAACTACATTCAATTCCAT-3′ | 199 bp |
| Reverse | 5′-ACCGATCCACACAGAGTACTTGCGC-3′ | |