Table 1

Effects of pertussis toxin on Cl-IBMECA-induced inhibition of cyclic AMP production in human ADF cells

cAMP Production
VehiclePertussis Toxin-Treated
pmol/min/mg
Basal61.8  ±  3.473.8  ±  6.3
Forskolin182  ±  11.2227  ±  14.3
Cl-IBMECA138  ±  9.81-150 212  ±  15.21-160

Confluent cells were pretreated with either 300 ng/ml pertussis toxin or vehicle for 12 h, and the effect of 100 nM Cl-IBMECA on forskolin-stimulated cAMP production was measured in membrane cell preparations as described under Materials and Methods. Data are presented as mean ± S.E.M. of three independent experiments.

    • ↵1-150 , p < 0.05 with respect to corresponding value of forskolin alone.

    • ↵1-160 , not statistically different from corresponding value of forskolin-stimulation of adenylyl cyclase alone.