Binding of [3H]tiotidine
| Cell Clones | Kd | Bmax |
|---|---|---|
| nM | sites/cell | |
| U-937 | 26 ± 3 (6) | 29,700 ± 1,600 (6) |
| D2 | 27 ± 4 (3) | 28,960 ± 1,432 (3) |
| A2 | 29 ± 5 (3) | 21,970 ± 1,143 (3)3-150 |
| D5 | 26 ± 4 (3) | 19,900 ± 900 (3)3-150 |
| H3 | 28 ± 5 (3) | 28,620 ± 1,829 (3) |
K d and maximal bound (B max) were calculated using the equation for one binding site. The table shows the mean ± S.E.M.; the number of determination (n) is in parentheses.K d and B max values were compared with U-937 using an unpaired t test, and the D5 and A2 clones were found to be significantly different.
↵3-150 , p < 0.01.