Effect of different signal transduction inhibitors on bFGF-induced cell proliferation, in the presence or absence of the MEK inhibitor PD 98059
| –PD98059 (10 μM) | +PD98059 (10 μM) | |
|---|---|---|
| bFGF (30 ng/ml) | 100 ± 8 | 28.4 ± 1** |
| bFGF + D609 (20 μM) | 70 ± 5.11-150 | 21 ± 1.9** |
| bFGF + NNA (1 μM) | 80 ± 31-150 | 17 ± 2.3** |
| bFGF + Ly-83583 (10 nM) | 71 ± 4.21-150 | 21.2 ± 2.1** |
| bFGF + KT 5823 (100 nM) | 70 ± 41-150 | 22 ± 3** |
Data are expressed as percentage of bFGF-induced [3H]thymidine incorporation. Basic FGF caused an increase of 130% over basal [3H]thymidine incorporation (p < 0.01).
↵1-150 , p < 0.05;**, p < 0.01 versus bFGF.