Table 1

Subunit stoichiometry determined from [35S]methionine incorporation

Subunit Incorporated [35S]Methionine Activity
Experimentα4β2Ratio (α/b)1-a
dpm
1
 Control243515241.6
 Cultured in nicotine1-b 523053261.0
2
 Control6825031.4
 Cultured in nicotine406137581.1

  • ↵1-a human α4 and β2 subunits contain the same number of methionine residues.

  • ↵1-b Nicotine (0.2 μM) was added along with [35S]methionine.