Potency and maximal activity (relative to adenosine) of adenosine-related agonists that elicited calcium responses from human immature MDDCs (DC) and hA3/G αq-i3/293 recombinant cells (R)
| Agonist | EC50 | Max | ||
|---|---|---|---|---|
| DC | R | DC | R | |
| nM | % | % | ||
| Adenosine | 18 (2–59) | 28 (21–36) | 100 | 100 |
| CGS21680 | 87 (21–175) | 81 (61–106) | 77 (24–103) | 98 ± 10 |
| IB-MECA | 0.8 (0.6–3.0) | 3 (2–5) | 49 (32–67) | 60 ± 5 |
| I-AB-MECA | 3 (1–7) | 3 (2–5) | 40 (32–56) | 55 ± 6 |
| 2Cl-IB-MECA | 7 (0.2–19) | 15 (6–25) | 25 (0–52) | 33 ± 6 |
MDDC values are means from at least five donors; range is given in parentheses. For recombinant cells, EC50 values (and in parentheses, 95% confidence interval) are representative of four independent experiments. Max values are means ± S.E.M. from four independent experiments.