Effects of wt and mutant p53 on topo IIα promoter constructs in (10)1 cells
| Topo IIα Construct | No. of ICBs | Relative Luciferase Activity | wt p53 | Mutant p53 |
|---|---|---|---|---|
| % of control | ||||
| pT IIα-32 | 0 | 1 | 147 ± 15 | 151 ± 14 |
| pT IIα-90 | 1 | 4.9 | 55 ± 8 | 82 ± 8 |
| pT IIα-142 | 2 | 12.8 | 25 ± 3 | 98 ± 10 |
| pT IIα-252 | 3 | 132 | 12 ± 1 | 62 ± 1 |
| pT IIα-382 | 4 | 202 | 11 ± 2 | 87 ± 23 |
(10)1 cells were cotransfected with topo IIα promoter constructs and either wt or mutant p53 expression vector. p53 values are expressed as a percentage of the luciferase activity in lysates from cells cotransfected with the control CMV-neo-Bam vector only. The values are the mean ± 1 S.D. of activities from triplicate samples in a representative experiment.