NeuriteExtension | |||
---|---|---|---|
Treatment | 24 h | 48 h | 72 h |
number/cm 2 | |||
Control | 1.4 ± 0.4 | 1.6 ± 0.8 | 1.9 ± 0.9 |
15-deoxy PGJ2 | |||
0.2 μM | 1.9 ± 0.7 | 2.3 ± 0.5 | 2.5 ± 1.3 |
0.4 μM | 2.4 ± 1.1 | 2.5 ± 1.4 | 3.7 ± 1.3 |
0.8 μM | 2.3 ± 1.4 | 2.9 ± 0.7 | 3.7 ± 0.8 |
1.6 μM | 2.6 ± 1.6 | 2.8 ± 0.6 | 3.2 ± 1.4 |
3.2 μM | 2.2 ± 0.9 | 2.2 ± 1.7 | 2.2 ± 1.5 |
NGF | |||
50 ng/ml | 6.8 ± 3.2 | 7.2 ± 1.1 | 9.8 ± 1.7 |
NGF + 15-deoxy PGJ2 | |||
0.2 μM | 10.3 ± 1.6 | 11.5 ± 1.3 | 13.1 ± 1.5 |
0.4 μM | 11.3 ± 0.9 | 14.1 ± 1.6 | 16.3 ± 1.3 |
0.8 μM | 14.2 ± 2.9 | 16.8 ± 1.6 | 18.8 ± 2.6 |
1.6 μM | 15.3 ± 2.7 | 16.3 ± 3.7 | 15.8 ± 3.7 |
3.2 μM | 13.5 ± 2.3 | 14.3 ± 2.1 | 13.1 ± 1.5 |
Troglitazone | |||
1 μM | 2.1 ± 0.3 | 2.5 ± 1.2 | 2.5 ± 1.5 |
5 μM | 1.9 ± 1.3 | 2.2 ± 1.7 | 2.6 ± 0.7 |
+ NGF | 7.9 ± 1.5 | 7.2 ± 2.7 | 8.4 ± 1.7 |
BADGE | |||
20 μM | 13.2 ± 2.6 | 16.3 ± 2.6 | 17.8 ± 1.9 |
50 μM | 15.3 ± 2.7 | 15.3 ± 3.9 | 16.2 ± 2.1 |
PC-12 cells were cultured with various doses of 15-deoxy PGJ2 in the absence or presence of NGF (50 ng/ml), or troglitazine alone for 72 h. Bisphenol A diglycidyl ether (BADGE) was pretreated 2 h before the treatment of 15-deoxy-PGJ2 with NGF. Neurite extension was assessed by measuring the number of neurite extension per unit area of culture as described under Materials and Methods. Values are mean ± S.E. of three experiments performed in triplicate.