Effect of various compounds on uptake of [3H]E1S by TR-CSFB cells
| Inhibitor | Concentration | C/M Ratio | % of Control |
|---|---|---|---|
| mM | μl/mg protein | ||
| Control | 10.8 ± 0.55 | 100 ± 5.04 | |
| E1S | 1 | 1.29 ± 0.07 | 11.9 ± 0.60** |
| Probenecid | 1 | 4.14 ± 0.34 | 38.3 ± 3.17** |
| Cholate | 1 | 6.44 ± 0.89 | 59.5 ± 8.21** |
| Taurocholate | 1 | 4.56 ± 0.22 | 42.1 ± 2.06** |
| BSP | 1 | 3.51 ± 0.57 | 32.5 ± 5.25** |
| DHEAS | 1 | 1.65 ± 0.24 | 15.3 ± 2.25** |
| T3 | 0.1 | 5.33 ± 0.41 | 59.1 ± 4.58** |
| T4 | 0.1 | 6.76 ± 0.18 | 75.0 ± 2.001-150 |
| Digoxin | 0.025 | 7.41 ± 0.17 | 68.5 ± 1.55** |
| PAH | 1 | 9.16 ± 0.32 | 84.6 ± 2.93 |
| Methotrexate | 0.1 | 10.6 ± 1.48 | 98.7 ± 13.7 |
| GABA | 1 | 10.6 ± 1.08 | 98.3 ± 9.97 |
[3H]E1S (115 nM) uptake by TR-CSFB cells was measured at 37° C for 5 min. Each value represents the mean ± S.E.M. (n = 3–20).
↵1-150 , P < 0.05; **, P < 0.01, significantly different from the control uptake.