Cx43 promoter | 5′-TCATCCTCCAAGGAGTTCAATCACTTGGCGTGACTTCACTACTTTTAAGCAA-3′ |
---|---|
FR3 | CCTCCAAGGAGTTCAATCACTTGGCGTGACTTCACTACTTTTA |
FR3-M1 | CCTCCAAGGAGTTCAATCACTGAGCGTGACTTCACTACTTTTA |
FR3-M2 | CCTCCAAGGAGTTCAATCACTTGGGAAGACTTCACTACTTTTA |
FR3-M3 | CCTCCAAGGAGTTCAATCGGGTGGCGTGACTTCACTACTTTTA |
FR3-M4 | CCTCCAAGGAGTTCAGGGACTTGGCGTGACTTCACTACTTTTA |
FR3-M5 | CCTCCAAGGAGTTCAATCACTTGGCGTCGGTTCACTACTTTTA |
FR3-M6 | CCTCCAAGGAGTGGGATCACTTGGCGTGACTTCACTACTTTTA |
FR3-M7 | CCTCCAGCCAGTTCAATCACTTGGCGTGACTTCACTACTTTTA |
FR3-B | TAAGCAAAAGAGTAAGTTTTTAAAAAATG |
The listed DNA oligonucleotides were designed by annealing as described under Materials and Methods and used to compete Cx200 in EMSAs. With the exception of FR3-B, they are homologous to the human Cx43 promoter, between positions +140 and +182, and contain the underlined base substitutions. FR3-B is homologous to the human Cx43 promoter, between positions +181 and +209. The promoter element binding to the transcription complex is shown in bold, and is referred to as RRCxE.